streptavidin fitc conjugate Search Results


fitc conjugated streptavidin antibody  (Rockland Immunochemicals)
93
Rockland Immunochemicals fitc conjugated streptavidin antibody
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Fitc Conjugated Streptavidin Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pmc07378189-163-3-10?v=Rockland+Immunochemicals
Average 93 stars, based on 1 article reviews
fitc conjugated streptavidin antibody - by Bioz Stars, 2026-07
93/100 stars
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horseradish peroxidase conjugated goat  (Rockland Immunochemicals)
85
Rockland Immunochemicals horseradish peroxidase conjugated goat
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Horseradish Peroxidase Conjugated Goat, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pmc02699996-99-14-21?v=Rockland+Immunochemicals
Average 85 stars, based on 1 article reviews
horseradish peroxidase conjugated goat - by Bioz Stars, 2026-07
85/100 stars
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hrp conjugated streptavidin  (Rockland Immunochemicals)
91
Rockland Immunochemicals hrp conjugated streptavidin
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Hrp Conjugated Streptavidin, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pmc07530788-106-3-15?v=Rockland+Immunochemicals
Average 91 stars, based on 1 article reviews
hrp conjugated streptavidin - by Bioz Stars, 2026-07
91/100 stars
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secondary fluorescein isothiocyanate fitc conjugated goat anti rabbit  (Rockland Immunochemicals)
85
Rockland Immunochemicals secondary fluorescein isothiocyanate fitc conjugated goat anti rabbit
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Secondary Fluorescein Isothiocyanate Fitc Conjugated Goat Anti Rabbit, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pmc03101819-154-2-9?v=Rockland+Immunochemicals
Average 85 stars, based on 1 article reviews
secondary fluorescein isothiocyanate fitc conjugated goat anti rabbit - by Bioz Stars, 2026-07
85/100 stars
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fitc-labeled streptavidin  (Becton Dickinson)
90
Becton Dickinson fitc-labeled streptavidin
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Fitc Labeled Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pmc03260167-135-11-12?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
fitc-labeled streptavidin - by Bioz Stars, 2026-07
90/100 stars
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texas red- or fitc-conjugated streptavidin  (Promega)
90
Promega texas red- or fitc-conjugated streptavidin
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Texas Red Or Fitc Conjugated Streptavidin, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pm18233996-105-10-12?v=Promega
Average 90 stars, based on 1 article reviews
texas red- or fitc-conjugated streptavidin - by Bioz Stars, 2026-07
90/100 stars
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fitc- or apc-conjugated streptavidin  (Becton Dickinson)
90
Becton Dickinson fitc- or apc-conjugated streptavidin
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Fitc Or Apc Conjugated Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pmc02292416-130-6-10?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
fitc- or apc-conjugated streptavidin - by Bioz Stars, 2026-07
90/100 stars
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fitc-conjugated streptavidin  (EuroBioSciences)
90
EuroBioSciences fitc-conjugated streptavidin
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Fitc Conjugated Streptavidin, supplied by EuroBioSciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pm22210908-53-16-14?v=EuroBioSciences
Average 90 stars, based on 1 article reviews
fitc-conjugated streptavidin - by Bioz Stars, 2026-07
90/100 stars
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streptavidin conjugated fluorescein isothiocyanate (fitc  (Biocan Diagnostics Inc)
90
Biocan Diagnostics Inc streptavidin conjugated fluorescein isothiocyanate (fitc
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Streptavidin Conjugated Fluorescein Isothiocyanate (Fitc, supplied by Biocan Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/10__1523_slash_jneurosci__18___07___02486__1998-59-29-37?v=Biocan+Diagnostics+Inc
Average 90 stars, based on 1 article reviews
streptavidin conjugated fluorescein isothiocyanate (fitc - by Bioz Stars, 2026-07
90/100 stars
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fitc-conjugated streptavidin  (Cosmo Bio USA)
90
Cosmo Bio USA fitc-conjugated streptavidin
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Fitc Conjugated Streptavidin, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/pm11777955-65-0-5?v=Cosmo+Bio+USA
Average 90 stars, based on 1 article reviews
fitc-conjugated streptavidin - by Bioz Stars, 2026-07
90/100 stars
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streptavidin-conjugated fitc  (Bangalore Genei)
90
Bangalore Genei streptavidin-conjugated fitc
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
Streptavidin Conjugated Fitc, supplied by Bangalore Genei, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/streptavidin+fitc+conjugate/bio_rxiv__2022__06__14__496079-104-4-9?v=Bangalore+Genei
Average 90 stars, based on 1 article reviews
streptavidin-conjugated fitc - by Bioz Stars, 2026-07
90/100 stars
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streptavidin biotinylated or fitc- or pe- or percp- conjugated  (Becton Dickinson)
90
Becton Dickinson streptavidin biotinylated or fitc- or pe- or percp- conjugated
a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated <t>streptavidin</t> beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.
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a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated streptavidin beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.

Journal: Cell Death & Disease

Article Title: Tim-4 functions as a scavenger receptor for phagocytosis of exogenous particles

doi: 10.1038/s41419-020-02773-7

Figure Lengend Snippet: a – c LR73 cells transfected with HA-Tim-4 were incubated with TAMRA-labeled apoptotic thymocytes, FITC-labeled E. coli , or S. aureus bioparticles for 2 h, extensively washed with ice-cold PBS, stained with anti-HA antibody, and observed using confocal microscopy ( a ) or flow cytometry ( b , c n = 3). TAMRA- or FITC-positive cells were considered to be phagocytes engulfing apoptotic thymocytes, or E. coli or S. aureus bioparticles, respectively. Yellow arrows indicate Tim-4-positive cells, and white arrows indicate Tim-4-negative cells. Scale bar, 10 µm. d , e Phagocytosis of red fluorescence-labeled polystyrene beads (carboxylate-modified beads) ( d n = 3) or zymosan A ( e n = 3) by LR73 cells transfected with HA-Tim-4 was analyzed as in ( b ). f LR73 cells stably expressing Tim-4 were incubated with the E. coli or S. aureus bioparticles for 2 h, and engulfing phagocytes were analyzed using flow cytometry ( n = 4). g The indicated cells were incubated with FITC-labeled IgG-coated streptavidin beads for 2 h and analyzed by flow cytometry. h LR73 cells stably expressing Tim-4 or control cells were transfected with Raichu-Rac1, and FRET was measured (99 cells for control-stable cells, 84 cells for Tim-4-stable cells). i Phagocytosis of the indicated targets by LR73 cells transfected with HA-Anxa5-GPI was evaluated as in ( b ) ( n = 3). Scale bar, 10 μm. j , k FITC-labeled E. coli ( j ) or S. aureus ( k ) bioparticles were intraperitoneally injected into WT or Tim-4 −/− mice, and then, 20 min after injection, the mice were sacrificed, and peritoneal exudates were stained with anti-F4/80 antibody. F4/80- and FITC-positive cells were considered to be phagocytes engulfing E. coli ( j four mice per each sample) or S. aureus ( k four mice per each sample) particles. All data are shown as the mean ± standard error of mean. Images are representative of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001. NS not significant, AC apoptotic cells.

Article Snippet: For IgG opsonization, FITC-conjugated streptavidin antibody (200-402-095) was purchased from Rockland Immunochemicals.

Techniques: Transfection, Incubation, Labeling, Staining, Confocal Microscopy, Flow Cytometry, Fluorescence, Modification, Stable Transfection, Expressing, Control, Injection